Prenatal sex determination is essential in the diagnosis of X-linked genetic disorders, such as hemophilia and Duchenne muscular dystrophy. Up to now, ways to prenatal sexing are CVS, Aminiocentesis, sono, and so on. But the above methods have
some
drawbacks like risks of abortion and possible induced congenital anomalies, difficulties to cell collection, culture and reading, a long waiting time, etc. According to the invention of PCR, it becomes possible to determine sex prenatally in the
maternal peripheral blood using fetal Y chromosome-specific ZFY gene DNA sequence amplification. Results of this study show all PCR positive bands in 24 cases of male-pregnant maternal peripheral blood and 23 among 26 cases of female-pregnant
maternal
peripheral blood show PCR negative bands from gestation 9 weeks to 41 weeks. 3 cases of female-pregnant maternal peripheral blood show PCR positive bands which probably from male cell contamination. With proper precautions, this method can be
used
to
determine the fetal sex prenatally in case of X-linked genetic disorders.
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